Modifying Enzymes
New England Biolabs, Inc. Immobilized T4 DNA Ligase - 1.1 mg
Immobilized T4 DNA Ligase (IM-T4 DNA Ligase) is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, enzyme can be removed using a magnet thereby avoiding heat inactivation, and can be re-used.
![Research Products International Corp DL-PHOSPHINOTHRICIN [PPT], 250 MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P40100-0.25.JPG-250.jpg)
Research Products International Corp DL-PHOSPHINOTHRICIN [PPT], 250 MG
Analog of Glutamate that acts as a competitive inhibitor of Glutamine synthetase. This enzyme is involved in assimilation of ammonia and plays a key role in nitrogen assimilation.
New England Biolabs, Inc. DNase I-XT-5000 units
An engineered variant of DNase I DNase I-XT is a salt-tolerant DNA endonuclease that nonspecifically cleaves DNA to release di- tri- and oligonucleotide products with 5 prime phosphorylated and 3 prime hydroxylated ends. DNase I-XT acts on single- and double-stranded DNA chromatin and the DNA strand of RNADNA hybrids. While DNase I (M0303) is inhibited by salt concentrations greater than 50 mM DNase I-XT exhibits optimal activity between 50-100 mM salt and retains 65% and about 40% activity in 200 and 300 mM salt respectively. This increased salt tolerance makes DNase I-XT the preferred enzyme for DNA template removal from an in vitro transcription (IVT) reaction. Importantly DNase I-XT is RNase-free allowing for the complete removal of DNA from RNA preparations while maintaining RNA integrity.
ACROBiosystems BenzNuclease DNA and RNA Nuclease (Ultra Pure,Protease Free)
Recombinant Protein;10KU;BenzNuclease-DNA-RNA-Nuclease (BEE-N3116) is expressed from E.coli cells.;BenzNuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. BenzNuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length.
![Research Products International Corp Cellulase RS [Onozuka RS], 1 Gram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C32400-1.0.JPG-250.jpg)
Research Products International Corp Cellulase RS [Onozuka RS], 1 Gram
From mutant Trichoderma viride derived from the parent strain for Cellulase Onozuka R-10. Cellulase RS contains a high activity of decomposing natural celluloses. Can be used to obtain protoplasts and dissolve cell walls in a wide range of plants.
Research Products International Corp Macerozyme R-10, 5 Grams
Macerating enzyme from Rhizopus sp. Often used in combination with Cellulase (C32200). Optimum pH range 3.5 - 7.0.
New England Biolabs, Inc. Msz Exonuclease I - 1000 units
A DNA specific exonuclease isolated from Methylocaldum szegediense that catalyzes the removal of nucleotides from linear single-stranded DNA in the 3 prime to 5 prime direction with optimal activity between 45 and 60 degrees C. Isolated from a strain of E. coli which carries the cloned pMC45-1 gene from Methylocaldum szegediense.
Enzo Life Sciences Matrix metalloproteinase-9 (MMP-9) fluorometric drug discovery kit, RED (1x96wells)
The MMP-9 Fluorometric (also known as fluorimetric) Drug Discovery Kit, RED is a complete assay system designed to screen MMP-9 inhibitors using a quenched fluorogenic substrate OMNIMMP RED: TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6-TAMRA)-Ala-Lys-NH2 [TQ3=quencher; GABA=4-aminobutyric acid; Cha=L-cyclohexylalanine; Abu=2-aminobutyric acid; Smc=S-methyl-L-cysteine; Dab=2,4-diaminobutyric acid; 6-TAMRA=6-tetramethylrhodamine]. TAMRA fluorescence is thoroughly quenched by the TQ3 group until cleavage by MMPs separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-9, a potential therapeutic target. The compound NNGH is also included as a prototypic control inhibitor. Alternative name: Gelatinase B, 92kDa Type IV collagenase. Applications: Fluorescent detection, HTS Activity assay.
New England Biolabs, Inc. Tth Argonaute (TtAgo) – 50 pmol
TtAgo is a prokaryotic argonaute from the Gram-negative, thermophilic bacterium Thermus thermophilus which functions as a DNA-guided endonuclease when provided with a 16-18 nucleotide long 5-phosphorylated single-stranded DNA oligonucleotide guide. The RNase H-like active site of TtAgo requires divalent Mg2+ (or Mn2+) metal ions for the reaction to occur and cleaves a complementary substrate between the bases corresponding to positions 10 and 11 of the DNA guide.
Enzo Life Sciences HDAC1 (human), (recombinant) (His-tag) (50 µg)
Alternative name: Histone deacetylase 1. Formulation: Liquid. In 50mM TRIS, pH 8.0, 138mM sodium chloride and 10% glycerol. Source: Produced in insect cells. HDAC1 from human cDNA (482 aa). Produced in a baculovirus expression system. Long Term Storage: -80°C.
New England Biolabs, Inc. RNase HII – 1250 units
Ribonuclease HII (RNase HII) is an endoribonuclease that preferentially nicks 5' to a ribonucleotide within the context of a DNA duplex. The enzyme leaves 5' phosphate and 3' hydroxyl ends. RNase HII will also nick at multiple sites along the RNA portion of an Okazaki fragment.
New England Biolabs, Inc. Salt-T4 DNA Ligase – 20000 units
A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase. This enzyme will join cohesive end termini at salt concentrations as high as 300 mM without any loss in activity. This enzyme is insensitive to salt carried over from other reaction components (vector or insert DNA) and allows ligation reactions to proceed in alternative reaction buffers with higher levels of salt (e.g., NEBuffer r3.1).
New England Biolabs, Inc. Sce PUS1 – 5000 pmol
Sce Pseudouridine Synthase I (Sce PUS1) is expressed as a 6X His tagged recombinant protein and converts Uridine to Pseudouridine in RNA. Sce PUS1 has a preference for Uridines in single stranded RNA regions over Uridines in double-stranded RNA. Optimal substrate is an unstructured RNA that is 15 nt long or longer. Sequence-specific pseudouridine modification by Sce PUS1 is an alternative to randomly incorporated modified nucleosides by RNA polymerases.